Description
A rapid chromatographic immunoassay for the qualitative detection of luteinizing hormone (LH) in urine as an aid in the detection of ovulation.
The test uses a combination of antibodies, including the LH monoclonal antibody, to selectively detect elevated LH levels.

How to use
Sample collection and preparation
Determine the optimal time for urine collection. For best results, collect urine at approximately the same time each day. Do not collect the first urine sample after waking up. Reduce fluid intake approximately 2 hours before urine collection.
Record the date, day of your period, and time of urine collection.
Urine can be stored at room temperature for up to 8 hours or at 2-8°C (36-46°F) for up to 24 hours. Do not freeze. For best results, test urine the same day it is collected. If refrigerated, allow urine to reach room temperature before testing.
Do not shake the container. If sediment forms at the bottom of the collection container, allow it to settle. Use only urine from the top of the container.

When to start the test
Calculate when to start testing using the table.
First, determine the length of your menstrual cycle. The length of your menstrual cycle is the number of days from the first day of your period (menstrual bleeding) to the last day before the start of your next period. Next, determine how many days from the start of your cycle you should count to begin the test. Find your menstrual cycle length on the first line of the table and read the corresponding number on the second line. This is the number of days you should count from the first day of your period.

Menstrual cycle length	21	22	23	24	25	26	27	28	29	30	31	32	33	34	35	36	37	38
Days to count ahead	6	6	7	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21

Running the test
Determine the day you will start testing using the "When to Start Testing" table.
Bring the pouch to room temperature before opening. Remove the midstream test from the sealed pouch and use it within one hour.
Remove the cap and hold the midstream test with the exposed absorbent tip facing down directly into your urine stream for at least 15 seconds until completely wet. Do not urinate on the results window. If you prefer, urinate into a clean, dry container and then immerse only the absorbent tip of the midstream test for at least 15 seconds.
After removing the midstream test from the urine, immediately replace the cap on the absorbent tip, place the midstream test on a flat surface with the result window facing up, and then begin counting.
When the test starts working, you may notice a light colored stream moving across the results window.
Read the results after 3 minutes. If no results appear, wait an additional minute. Do not read the results after 10 minutes.

Reading the results
POSITIVE: Two colored lines are visible and the line in the test zone (T) is equal to or darker than the line in the control zone (C). This indicates that ovulation will likely occur within the next 24-36 hours.

NEGATIVE: Two colored lines are visible, but the line in the test region (T) is lighter than the line in the control region (C), or there is no line in the test region (T). This indicates that no LH surge has been detected and that daily testing should continue.

INVALID: The result is invalid if no colored line appears in the control region (C), even if a colored line appears in the test region (T). It is advisable to repeat the procedure with a new test. If the problem persists, discontinue use of the test kit immediately and contact your local distributor.

Warnings
For in vitro self-diagnosis. Not for internal use.
Before performing the test, read all the information in the package leaflet.
Do not use after the expiry date printed on the package.
Do not use if pouch is torn or damaged.
Keep out of reach of children.
Do not open the foil pouch until you are ready to begin testing.
The used test must be disposed of according to local regulations.
The test cannot be used as a form of birth control.
Test results should not be affected by painkillers, antibiotics, or other common medications. Medications containing hCG or LH may affect the test and should not be taken while using the test. Additionally, the test will not work properly for women who are pregnant, going through menopause, or taking birth control pills.

Conservation
Store in a dry place at 2-30°C. Do not freeze.
Shelf life in intact packaging: 24 months.

Format
The kit contains:
- Midstream testing;
- package leaflet.

Cod. SF001